Coding

Part:BBa_K5466031:Design

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-24)


AntiAFB1-scFv1 EpoR Split-N-mCerulean


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to use a Bimolecular Fluorescence Complementation (BiFC) system because our receptor is based on split-ubiquitin, so we thought it was the best option to verify that the scFvs used in our project can dimerize in the cytoplasmatic membrane. We also used the same signal peptide to keep the conditions as similar as possible


Source

Source in each individual part.